Abstract
Drug delivery is a crucial aspect of pharmaceutical research and is a vital issue to address when aiming to formulate effective therapies. 5-Fluorouracil (5-FU) is one of the most prescribed small molecule drugs for the treatment of several tumors (e.g., colorectal, breast, liver and pancreatic cancer).
While numerous approaches have been proposed to minimize the side effects of 5-FU, drug delivery nanosystems, such as liposomes, have shown the most promising results in improving 5-FU’s therapeutic efficiency.
The main goal of the NanoCanTune project is to investigate liposome stability, drug release properties and liposome-protein interactions in a biological environment in view of optimizing liposomal formulations for generating improved 5-FU delivery nanosystems.
These features will be investigated for the first time by a combined computational and proteomic approach. It is expected that the obtained results will provide valuable information for fine-tuning the composition of liposomal nanosystems containing 5-FU.
Project coordinator: CSIII Dr. Pomohaci Roxana-Maria
General information
|
Financial resource |
State budget |
|
PNCDI III: |
Program 1 - Development of the National R&D system |
|
Project type: |
Postdoctoral Research Project |
|
Code: |
PN-III-P1-1.1-PD-2021-0786 |
|
Contract no: |
PD 66/13.04.2022 |
|
Project value: |
250,000 lei |
|
Duration: |
24 months (01.04.2022 – 31.03.2024) |
|
Project authority: |
The Executive Agency for Higher Education, Research, Development and Innovation Funding |
|
Contractor: |
The Regional Institute of Oncology Iasi |
|
Project coordinator: |
CSIII Dr. Pomohaci Roxana-Maria |
Objective
Investigation of key aspects of drug delivery systems through a combined synthetic, computational and proteomic approach in view of optimizing liposomal nanoformulations containing the anticancer agent 5-FU.
Financial resources
|
NO |
Category |
2022
(lei) |
2023
(lei) |
2024
(lei) |
TOTAL
(lei) |
|
1 |
Staff expenses |
35,910.00 |
62,340.00 |
15,270.00 |
113,520.00 |
|
2 |
Logistics |
26,450.00 |
49,813.00 |
0 |
76,263.00 |
|
2.1 |
Mobilities |
0 |
9,275.00 |
9,275.00 |
18,550.00 |
|
2.2 |
Indirect costs |
12,472.00 |
24,286.00 |
4,909.00 |
41,667.00 |
|
|
TOTAL |
74,832.00 |
145,714.00 |
29,454.00 |
250,000.00 |
Work plan
|
Year |
Work plan |
|
2022 |
Liposomal nanovehicle design, development and physico-chemical characterization
-
Develop a protocol for liposome formulation, drug encapsulation and investigation of loading capacity and encapsulation efficiency
-
Physico-chemical characterization of novel liposomal formulations of 5-FU
|
|
2023 |
Liposomal nanovehicle stability and behavior in a biological environment:
-
Investigation of the stability of liposomal formulations and drug distribution within the nanocarrier by molecular dynamics simulations
-
Investigation of novel liposomal formulations’ behavior in rat plasma in terms of protein corona formation
|
|
2024 |
Behavior of liposomal nanovehicles in a biological environment:
-
Characterization of formed supramolecular assemblies
|
Communication and dissemination
-
A. Publications
-
B. Scientific Conferences
-
Investigating plasma protein adsorption for 5-fluorouracil-encapsulating liposomes, R-.M. Amărandi, L. Marin, B. Dragoi; NanoBio&Med2022, 22-24.11.2022, Barcelona, Spania – poster
-
C. Scientific report - see report
-
A. Publications
-
B. Scientific Conferences
-
Designing liposomal formulations of 5-fluorouracil for cancer therapy, R-.M. Amărandi, L. Marin, A. Neamțu, B. Dragoi; Congresul internațional al Universităţii Apollonia din Iaşi, “Pregătim viitorul, promovând excelența” ediţia a XXXIII-a, 02-05.03.2023, Iași, Romania – poster
-
How lipid composition impacts vesicle protein adsorption – a combined in silico and in vitro investigation, R-.M. Amărandi, L. Marin, A. Neamțu, B. Dragoi; NanoTech Poland 2023, 14-16.06.2023, Poznań, Poland – oral presentation
-
C. Scientific report
-
A. Publications
- The impact of lipid composition on vesicle protein adsorption. A BSA case study, R.-M. Amarandi, A. Neamtu, R.-I. Știufiuc, L. Marin, B. Dragoi, 2024, ACS Omega, accepted for publication (DOI: 10.1021/acsomega.3c09131)
- A coarse-grained molecular dynamics perspective on the release of 5-fluorouracil from liposomes, R.-M. Amarandi, L. Marin, B. Dragoi, A. Neamtu, 2024, Molecular Pharmaceutics, under review
- Maisine-based Microemulsions as Convenient Carriers for co-Loading of Anticancer and Anti-inflammatory Drugs. A Multi Spectroscopic Investigation, M. Nistor, A. Nicolescu, R.-M. Amarandi, A. Pui, R-I. Stiufiuc, B. Dragoi, 2024, Scientific Reports, under review
-
B. Scientific Conferences
- The impact of lipid composition on the adsorption of proteins onto liposomal vesicles, R.-M. Amarandi, A. Neamtu, R.-I. Știufiuc, L. Marin, B. Dragoi; Congresul internațional al Universităţii Apollonia din Iaşi, “Pregătim viitorul, promovând excelența” ediţia a XXXIV-a, 29.02-03.03.2024, Iași, Romania – poster
-
C. Final Scientific Report - see report
Research Team
CSIII Dr. Pomohaci Roxana-Maria
Project director
CSI Dr. habil. Marin Luminița
Mentor
Contact
CSIII Dr. Pomohaci Roxana-Maria
Regional Institute of Oncology Iasi
TRANSCEND Research Centre
2-4 General Henri Mathias Berthelot Street, Iași 700483
E-mail: rpomohaci@iroiasi.ro
